Related Studies

SEVENS ROOTS is an active complex with sexual stimulant effect for men and women, elaborated from a standardized powder extract of seven roots in different proportions, chuchuwasi (Maytenus macrocarpa), male hunchpo (Jatropha macrantha), black maca (Lepidium meyenii) , icoja (Unonopsis floribunda), murure (Brosimum acutifolium), cumaceba (Swartzia polyphyla), clove huasca (Tynanthus panurensis) and Inspired by the “tunche”, demon of Amazonian mythology, known for its seduction and virility.

All our DEVILS JUNGLE line, has as an active principle our SEVENS ROOTS active complex, this asset and all our products have been subjected to different tests that ensure their effectiveness and the innocuousness of their consumption.

DEVILS JUNGLES CAPSULES FOR MEN

DEVILS JUNGLE CAPSULAS FOR WOMEN

DEVILS JUNGLE INSTANT BEVERAGE FOR MEN

ANDRO VITALITY SMOOTHIE FOR ANDROPAUSE

1. CHARACTERIZATION AND STANDARDIZATION OF INPUTS (see technical report)

Activity 1.1: Determination of chemical and nutritional composition chemical, nutritional, physicochemical and phytochemical evaluation.

Activity 1.2: Physicochemical analysis (Determination of humidity, determination of ashes, determination of pH.)
Moisture determination.
Determination of PH.
Ash determination method AOAC 15.016 1980- gravimetric by incineration.

Activity 1.3: Phytochemical analysis
It will be carried out using the microchemical methodology, which was complemented with thin layer chromatography on Silica gel 60 F254 with aluminum support.

Activity 1.4: Microbiological analysis
In the determination of mesophilic aerobes (AOAC 990.12, AOAC 989.10) and enterobacteria (AOAC 2003.01) salmonella (Salmonella Express System – AOAC 2014.01) and molds and yeasts (AOAC official method 997.02).

2. COMPONENT 2: PRODUCT ELABORATION (see technical report)

Activity 2.1: Formulation of the final product
Pilot lots of 10 kg each were produced. To which their nutritional composition was evaluated, to determine which has the best profile.

Activity 2.2: Determination of chemical and nutritional composition
It will be determined, following the recommendations of the AOAC, the content of protein, total ash, fat, dietary fiber using the method enzymatic-gravimetric, total and reducing sugars and the content of minerals.

Activity 2.3: Physico-chemical analysis
Determination of humidity, determination of ashes, determination of pH.

Activity 2.4: Microbiological analysis
Determination of mesophilic aerobes and enterobacteria, salmonella, molds and yeasts according to official AOAC methods.

Activity 2.5: Sensory evaluation
50 older adults will be surveyed who will be presented with the optimized sample through MSR and a questionnaire.

3. DETERMINATION OF STABILITY DURING STORAGE (SHELF LIFE) (see technical report)

The analysis of useful life will be carried out considering the conservation and storage of the product in HDPE bottles, at room temperature (28+ 2ºC and 70% relative humidity). The accelerated life study will be carried out by means of the conservation of the samples at storage temperature 37 ° C ± 2 and it will be considered that 3 days in this temperature condition is equivalent to 7 days of storage at 28 ° C ± 2 (room temperature).

4. PHYTOCHEMICAL, ANTIOXIDANT EVALUATION (see technical report)

Activity 3.1: Phytochemical characterization
The main phytochemical components present in the final product will be identified through qualitative analysis and thin layer chromatography (TLC).

Activity 3.3: Characterization of the antioxidant activity ORAC method (Oxygen radical absorbance capacity).

5. PHARMACOLOGICAL EVALUATION OF THE PRODUCT (see technical report)

Given the difficulty of applying clinical evaluations, it has been decided to apply pre-clinical evaluations that will consist of:

See technical report

The effect of drug testing on mating behavior has been studied in the terms of the methods by Dewsbury and Davis Jr, modified by us. Relationships with sex and sexual experience have been selected for the study. They were divided into five groups, each of six rats separately and placed in separate propylene cages during the experiment. Group 1 served as a control group and received 10 ml / kg of distilled water orally, daily for 7 days at 18:00 h. Groups of 2 to 4 hours of suspension of the extraction orally at doses of 100, 250 and 500 mg / kg, respectively, once a day for 7 days at 18:00 h. Group 5 served as the standard group and given the suspension of the drug standard 1 h before the start of the experiment. The males should not be tested in unknown circumstances the animals were brought in the laboratory and in the weak light, it is established at the time of the daily tests for 6 days before the experiment.

Pairing of female rats was allowed only during the “estrus” phase. Thus, they were artificially induced in heat by the method of Szechtman et al. The oral ethinylestradiol suspension is administered in the dose of 100 μg / animal 48 hours before the pairing plus progesterone injected subcutaneously, at the dose of 1 mg / animal 6 h before the test. The receptivity of the females was confirmed before the test exposes them to male animals, other than the control, of tests and standard animals. The most receptive females were selected for the study. The experiment was carried out on the 7th day after the beginning of the treatment of the male animals. The experiment was carried out at 20:00 h in the same laboratory and with the same intensity of light. The receptive females were introduced into the cages of the male animals and 1 female of 1 male. The observation of the mating behavior began immediately and continued during the first 2 mating series. The test was terminated when the males showed no sexual interest. If the females did not show receptivity, they are replaced by another induced artificially. The occurrence of the events and the mating phases were recorded. Later, the frequencies and phases will be determined from the transcriptions of the recording: number of mounts before ejaculation or assembly frequency (FM), the number of intromissions before ejaculation or Intrusion Frequency (IF), time of the introduction of the words in the cage of the males until the first mount or montage of latency (ML), the time of the introduction of the female until the first intromissions by man or intrusion latency (IL), the time of the first one series of intrusions until ejaculation or ejaculatory latency (EL), and time of the first ejaculation until the next intromissions by man or Post Ejaculatory Interval (PEI). In the second series of mating only refers to EL. The values observed for the parameters of the control regimens, the standard tests and the animals were analyzed statistically by means of a variance analysis route (ANOVA).

See technical report

The test was carried out by the Davidson method, modified by us. The sexually experienced male rats were divided into five groups, each of six rats separately and kept in separate propylene cages during the experiment. Group 1 represents the control group, which received 10 ml / kg of distilled water orally, once a day for 7 days at 18:00 h. Group of 2-4 received suspension of oral extraction at doses of 100, 250 and 500 mg / kg, respectively, daily for 7 days at 18:00 h. Group 5 served as a standard group and given the suspension of the oral drug standard at a dose of 5 mg / kg, 1 h before the start of the test. The rats were made receptive by hormonal treatment and all the animals were accustomed to the condition of the aforementioned tests in the mating behavior test. The animals were observed for frequency assembly (FM) on the night of the 7th day, at 20:00 h. The penis was exposed by retracting the pod and 5% xylocaine ointment was applied 30, 15 and 5 minutes before commencing observations. Each animal was placed in an individual cage and the receptive female rat was placed in the same cage. The number of frames was noted. The animals were also observed for intromissions and ejaculation. The MF in the control, testing and standard animals was statistically analyzed by using one-way analysis of variance by the method (ANOVA).

See technical report

The test was carried out by the methods of Haugen and Hart and Hart, modified by us. The male rats were divided into five groups, each of six rats separately and placed in separate propylene cages during the experiment. Group 1 represents the control group, which received 10 ml / kg of distilled water orally daily for 7 days. Group of 2-4 received oral drug test suspension at the dose of 100, 250 and 500 mg / kg, respectively, daily for 7 days. Group 5 served as a standard group and received the suspension of the oral drug standard at the dose of 5 mg / kg, 1 h before the start of the test. At 8 days, the penile reflex test was carried out by placing the rat on the back, in a partial retention glass cylinder. The preputial sheath was pushed behind the head of the penis, by means of the thumb and forefinger and kept in this way for a period of 15 min. This stimulation causes a series of genital reflexes. The following components were recorded: Erections (E), Quick Flips (QF) and Long Flips (LF). The frequency of these parameters observed in the control, trial and group was statistically norm analyzed by the use of one-way analysis in the variance (ANOVA) method.

See technical report

In all treated rats, any signs of toxicity (salivation, rhinorrhea, lachrymation, ptosis, writing, tremors and convulsions), stress (skin erection and exophthalmia), and changes in the skin were observed at least once a day. behavior (such as spontaneous movement in the cage, climbing, cleaning the face). In addition, the intake of food and water were recorded.

See technical report

The acute toxicity of the extract was studied in adult albino mice of both sexes. They were divided into five groups, each of six mice. The suspension of the extract is administered orally in four different doses of 500, 1000, 2000 and 4000 mg / kg, respectively, to the different groups of mice separately. The controls received 10 ml / kg of distilled water orally. Animals were continuously observed for the initial 4 h of behavioral changes and mortality and intermittently for the next 6 h and then again at 24 h and 48 h after administration. The behavior of the parameters observed were convulsions, hyperactivity, sedation, cleanliness, loss of reflex and increase in respiration.

Devil Jungle